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Evidence Guide: MSS024033 - Identify environmentally significant organisms

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSS024033 - Identify environmentally significant organisms

What evidence can you provide to prove your understanding of each of the following citeria?

Obtain biological samples

  1. Locate and read site or project information to determine background and context of work
  2. Review work request to identify required samples and procedures, materials and equipment to be used
  3. Identify hazards and workplace control measures associated with collecting samples, preparation methods, reagents and equipment
  4. Assemble required materials, reagents and equipment and check they are fit for purpose
  5. Collect samples in accordance with legislative requirements, codes of practice and workplace procedures
  6. Log samples using workplace procedures
  7. Record sample description, compare with specification and note and report any discrepancies
  8. Apply traceability protocols from receipt of sample to reporting of results
Locate and read site or project information to determine background and context of work

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Review work request to identify required samples and procedures, materials and equipment to be used

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify hazards and workplace control measures associated with collecting samples, preparation methods, reagents and equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assemble required materials, reagents and equipment and check they are fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Collect samples in accordance with legislative requirements, codes of practice and workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Log samples using workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record sample description, compare with specification and note and report any discrepancies

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Apply traceability protocols from receipt of sample to reporting of results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare samples and grow cultures

  1. Use aseptic techniques to prepare media for microbiological analysis
  2. Prepare samples for microbiological analysis according to procedures
  3. Identify contamination pathways for both samples and media and apply relevant controls
  4. Perform simple chemical tests to identify morphology of environmentally significant bacteria
  5. Use aseptic techniques to manipulate samples and bacterial cultures
  6. Use standard techniques to grow cultures of environmentally significant microorganisms
  7. Use disinfection and sterilisation to control the growth of microorganisms
Use aseptic techniques to prepare media for microbiological analysis

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare samples for microbiological analysis according to procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify contamination pathways for both samples and media and apply relevant controls

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform simple chemical tests to identify morphology of environmentally significant bacteria

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use aseptic techniques to manipulate samples and bacterial cultures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use standard techniques to grow cultures of environmentally significant microorganisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use disinfection and sterilisation to control the growth of microorganisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare microscope for use

  1. Select microscope and attachments for examination and measurements to meet work request
  2. Check that all microscope components are clean and fit for purpose
  3. Mount sample in accordance with specified method
  4. Adjust settings and alignment of optical and mechanical components to optimise performance
Select microscope and attachments for examination and measurements to meet work request

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Check that all microscope components are clean and fit for purpose

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Mount sample in accordance with specified method

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Adjust settings and alignment of optical and mechanical components to optimise performance

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform microscopic examination in accordance with test methods

  1. Identify microorganisms, cell structures and components of animal and plant tissues in biological samples
  2. Measure organisms and structures
  3. Enumerate microorganisms in biological samples
  4. Select and use dichotomous key to classify organisms into hierarchical structures
  5. Record all observations and measurements used to determine classifications according to workplace procedures
Identify microorganisms, cell structures and components of animal and plant tissues in biological samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Measure organisms and structures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Enumerate microorganisms in biological samples

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select and use dichotomous key to classify organisms into hierarchical structures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record all observations and measurements used to determine classifications according to workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Determine environmental significance of organisms

  1. Identify cell structures of organisms
  2. Associate biological functions with cell structures
  3. Associate cell physiology and enzyme cycles to environmental adaptation relevant to site or project
  4. Identify important biochemical pathways associated with environmentally significant organisms
Identify cell structures of organisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Associate biological functions with cell structures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Associate cell physiology and enzyme cycles to environmental adaptation relevant to site or project

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Identify important biochemical pathways associated with environmentally significant organisms

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Use personal protective clothing and safety equipment to ensure safety and minimise cross-contamination
  2. Handle all samples and equipment in accordance with workplace safety procedures
  3. Clean up spills using techniques to protect personnel, work area and environment
  4. Minimise generation of waste and environmental impacts
  5. Collect and dispose of all waste safely
  6. Report hazards and incidents to designated personnel using workplace procedures
Use personal protective clothing and safety equipment to ensure safety and minimise cross-contamination

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Handle all samples and equipment in accordance with workplace safety procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Clean up spills using techniques to protect personnel, work area and environment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Minimise generation of waste and environmental impacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Collect and dispose of all waste safely

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Report hazards and incidents to designated personnel using workplace procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain workplace records

  1. Record approved data into workplace system
  2. Maintain confidentiality and security of workplace information and data
Record approved data into workplace system

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain confidentiality and security of workplace information and data

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Required Skills and Knowledge